Resource - Bioimaging and Biophotonics Laboratory

장비 사용 문의

Nikon’s Ti2-E platform with laser lines of 405/488/560/642 nm
Objectives: 100x/1.45 (oil), 100x/1.49 (oil, TIRF), 60x/1.27 (water), 20x/0.75
Camera: EMCCD (Andor’s iXon Ultra 888; 1024×1024 pixels)
Image acquisition: NIS-Elements AR or Home-built software
Achievable imaging resolution
– dSTORM: ~25/50 nm (xy/z) with organic dyes (Alexa Fluor 647, CF568, etc.)
– PALM: ~40/100 nm (xy/z) with photoconvertible fluorescent proteins

Laser diode system: 405 nm CW, 50 mW (OBIS 405 LX 50 mW, Coherent)
DPSS laser 405 nm CW, 250 mW (LSR405NL-250)
DPSS laser 405 nm CW, 500 mW (LSR405CPD-500)
DPSS laser: 488 nm CW, 200 mW (Sapphire 488-200 OEM, Coherent)
DPSS laser: 488 nm CW, 200 mW (Sapphire 488 HP, Coherent)
Fiber laser: 560 nm CW, 1.0 W (2RU-VFL-P-1000-560-B1R, MPB Communications)
Fiber laser: 642 nm CW, 1.0 W (2RU-VFL-P-1000-642-B1R, MPB Communications)
Fiber laser: 642 nm CW, 300 mW (VFL-P-300-642-OEM1-B1R, MPB Communications)
Ti:Sapphire femtosecond laser: 680-1080 nm (tunable), 140 fs pulses at 80 MHz, GDD compensation (Chameleon Vision II, Coherent)
Superluminescent diode: 880 nm, SM fiber output (SLD880S-A25 + CLD1015, Thorlabs)

sCMOS camera (Andor’s Sona 4.2B-6, SONA-4BV6U): 2048×2048 pixels, 6.5 µm pixel size, >95% peak QE, 43 fps (12/16 bit; USB 3.0)
sCMOS camera (Photometrics’ Prime BSI express): 2048×2048 pixels, 6.5 µm pixel size, >95% peak QE, 43 fps @16 bit (or 95 fps @11 bit; USB 3.2)
sCMOS camera (pco.panda 4.2): 2048×2048 pixels, 6.5 µm pixel size, >80% peak QE, 40 fps (16 bit; USB 3.1 Gen 1)
sCMOS camera (pco.edge 4.2 bi): 2048×2048 pixels, 6.5 µm pixel size, >95% peak QE, 40 fps (16 bit; USB 3.1 Gen 1), air/water cooling (-25°C)
EMCCD camera (Andor’s iXon Ultra 888, DU-888U3-CS0-BV): 1024×1024 pixels, 13 µm pixel size, >95% peak QE, 26 fps (16 bit; USB 3.0)
CMOS camera (Basler’s a2A4504-18umBAS): 4504×4504 pixels, 2.74 µm pixel size, 66% peak QE, 18 fps @12 bit)

AOTF (97-03151-01, G&H): CA 2.5 mm, 400-650 nm, 55-110 MHz
SLM (PLUTO-2-VIS-096, Holoeye Photonics AG): Reflective LCOS (phase only), 1920×1080 pixels, 8 µm pixel pitch, ~2π phase range (@650 nm; 8bit), 95~98% reflectivity (dielectric mirror coating, 450-650 nm)
Ferroelectric Liquid Crystal on Silicon (FLCoS) display (2K 0.94″ M180 R11, Kopin): reflective type, binary modulation (amplitude/phase), 2048×2048 pixels (0.94″), 8.2 µm pixel pitch, 55% reflectance, max 75 FPS
DMD (E4750RGBLC, EKB Technologies): 1920×1080 pixels (0.47″), 5.4 µm mirror pitch, 1.44 kHz rate (binary), visible (420-700 nm)
Deformable mirror (DMH40/M-P01, Thorlabs): 40 actuator array, Ø14.0 mm pupil diameter, protected sliver coating (0.45-20 µm), 4 kHz rate (USB2.0)

PIFOC objective scanning system (PD72Z2CA0, Physik Instrumente): 250 µm travel, 1.5 nm resolution (closed loop)
PIFOC objective scanning systems (Physik Instrumente): 400 µm travel, closed loop control
PIFOC objective scanning systems (Physik Instrumente): __0 µm travel, closed loop control

Hand-held refractometer (PAL-RI, Atago): refractive index from 1.3306 to 1.5284
Optical Microscopy Educational Kit (EDU-OMC1/M, Thorlabs)
Compact spectrometer (CCS200/M, Thorlabs): 200-1000 nm (wavelength range), <2 nm FWHM (@633 nm, spectral accuracy)
Digital oscilloscope (MSO5104, Rigol): 100 MHz BW, 4 CHs (Analog), 8 GSa/s, 2 CHs (Func. Gen.)
CO2 incubator (Heraeus BB15, Thermo Scientific): 150 L
Autoclave (VS-1321-60, Vision Science): 60 L capacity (∅300 mm × 630 mm), temp up to 130°C (2.4 kW heater), 95 kg weight
Micro centrifuge (LZ-1730R, Labogene): max 17,000 rpm/27,237 × g, temp range -20~4o°C, fixed angle rotor (24 × 1.5/2.0 mL, GREB-M-m2.0-24)
Rocker (DH.WRK01010, DAIHAN Medical): 290 × 200 mm platform, RK-1D, 5-50 rpm speed range
Western blotting systems (Bio-Rad): 4-gel vertical electrophoresis system (#1658001), mini trans-blot module (#1703935), power supply (#1645050)